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phosphorylated pka  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc phosphorylated pka
    Phosphorylated Pka, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 194 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phosphorylated+pka/Phospho-PKA+C+(Thr197)+Rabbit+mAb/pmc13011214-154-14-39
    Average 95 stars, based on 194 article reviews
    phosphorylated pka - by Bioz Stars, 2026-09
    95/100 stars

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    Incubation:

    Article Title: PKA and PKC signaling pathways mediate vitamin D₃-regulated intestinal phosphorus absorption in broiler chickens
    Article Snippet: .. PVDF membranes were incubated with primary antibodies against NaPi-IIb (1:1000, A9460; ABclonal, Wuhan, China), phosphorylated PKA (p-PKA; 1:1000, 5661S), phosphorylated PI3K (p-PI3K; 1:1000, 4228), phosphorylated ERK (p-ERK; 1:1000, 9101S), phosphorylated p38MAPK (p-p38MAPK; 1:1000, 9216S), total PKA (t-PKA; 1:1000, 5842S) (Cell Signaling Technology, Boston, USA), total PI3K (t-PI3K; 1:10000, 60225-1-Ig), total ERK (t-ERK; 1:8000, 11257-1-AP-50), total p38MAPK(t-p38MAPK; 1:1000, 14064-1-AP), total PKC (t-PKC; 1:1000, 21991-1-AP), phosphorylated PKC (p-PKC; 1:5000, 29123-1-AP), and GAPDH (1:10000, 60004-1-Ig) (Proteintech, Wuhan, China). .. After the membranes were washed, they were incubated with diluted secondary antibody horseradish peroxidase ( HRP )-labeled goat anti-rat IgG (1:5000; SA00001-1, Proteintech, Wuhan, China) at 37°C for 1 h. Immunoreactive bands were detected using a chemiluminescent imaging system (OI-X6Touch, Guangzhou, China).

    Article Title: S-allyl Cysteine Enhances Testosterone Production in Mice and Mouse Testis-Derived I-10 Cells
    Article Snippet: .. The membranes were then incubated overnight with blocking buffer containing an antibody against PKA, and phosphorylated PKA (Cell Signaling Technology, Danvers, MA, USA), followed by incubation for 1 h with horseradish peroxidase (HRP)-tagged secondary antibody. .. Antibodies against α-tubulin (Sigma-Aldrich) were incubated for 1 h followed by HRP-tagged secondary antibody.

    Article Title: PKA and PKC signaling pathways mediate vitamin D₃-regulated intestinal phosphorus absorption in broiler chickens.
    Article Snippet: .. PVDF membranes were incubated with primary antibodies against NaPi-IIb (1:1000, A9460; ABclonal, Wuhan, China), phosphorylated PKA (p-PKA; 1:1000, 5661S), phosphorylated PI3K (p-PI3K; 1:1000, 4228), phosphorylated ERK (p-ERK; 1:1000, 9101S), phosphorylated p38MAPK (p-p38MAPK; 1:1000, 9216S), total PKA (t-PKA; 1:1000, 5842S) (Cell Signaling Technology, Boston, USA), total PI3K (t-PI3K; 1:10000, 60225-1-Ig), total ERK (t-ERK; 1:8000, 11257-1-AP-50), total p38MAPK(t-p38MAPK; 1:1000, 14064-1-AP), total PKC (t-PKC; 1:1000, 21991-1-AP), phosphorylated PKC (p-PKC; 1:5000, 29123-1-AP), and GAPDH (1:10000, 60004-1-Ig) (Proteintech, Wuhan, China). .. After the membranes were washed, they were incubated with diluted secondary antibody horseradish peroxidase (HRP)-labeled goat anti-rat IgG (1:5000; SA00001-1, Proteintech, Wuhan, China) at 37◦C for 1 h. Immunoreactive bands were detected using a chemiluminescent imaging system (OI-X6Touch, Guangzhou, China).

    Article Title: S -allyl Cysteine Enhances Testosterone Production in Mice and Mouse Testis-Derived I-10 Cells
    Article Snippet: .. The membranes were then incubated overnight with blocking buffer containing an antibody against PKA, and phosphorylated PKA (Cell Signaling Technology, Danvers, MA, USA), followed by incubation for 1 h with horseradish peroxidase (HRP)-tagged secondary antibody. .. Antibodies against α-tubulin (Sigma-Aldrich) were incubated for 1 h followed by HRP-tagged secondary antibody.

    Blocking Assay:

    Article Title: S-allyl Cysteine Enhances Testosterone Production in Mice and Mouse Testis-Derived I-10 Cells
    Article Snippet: .. The membranes were then incubated overnight with blocking buffer containing an antibody against PKA, and phosphorylated PKA (Cell Signaling Technology, Danvers, MA, USA), followed by incubation for 1 h with horseradish peroxidase (HRP)-tagged secondary antibody. .. Antibodies against α-tubulin (Sigma-Aldrich) were incubated for 1 h followed by HRP-tagged secondary antibody.

    Article Title: S -allyl Cysteine Enhances Testosterone Production in Mice and Mouse Testis-Derived I-10 Cells
    Article Snippet: .. The membranes were then incubated overnight with blocking buffer containing an antibody against PKA, and phosphorylated PKA (Cell Signaling Technology, Danvers, MA, USA), followed by incubation for 1 h with horseradish peroxidase (HRP)-tagged secondary antibody. .. Antibodies against α-tubulin (Sigma-Aldrich) were incubated for 1 h followed by HRP-tagged secondary antibody.



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    Image Search Results


    Immunohistochemical Staining Results Immunohistochemical staining and analysis of osteogenic-related proteins and signaling pathway markers in distraction areas at consolidation weeks 2, 4 and 6. Staining for Runx2, Osterix (Osx), osteopontin (OPN), osteocalcin (OCN), phosphorylated PKA (p-PKA), and phosphorylated CREB (p-CREB) across different treatment groups. Quantitative analysis shows positive staining area percentage (n = 3). Scale bar: 200 μm.

    Journal: Materials Today Bio

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    doi: 10.1016/j.mtbio.2025.102181

    Figure Lengend Snippet: Immunohistochemical Staining Results Immunohistochemical staining and analysis of osteogenic-related proteins and signaling pathway markers in distraction areas at consolidation weeks 2, 4 and 6. Staining for Runx2, Osterix (Osx), osteopontin (OPN), osteocalcin (OCN), phosphorylated PKA (p-PKA), and phosphorylated CREB (p-CREB) across different treatment groups. Quantitative analysis shows positive staining area percentage (n = 3). Scale bar: 200 μm.

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    Techniques: Immunohistochemical staining, Staining