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phosphorylated pka  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc phosphorylated pka
    Phosphorylated Pka, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 182 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phosphorylated+pka/pmc13011214-154-14-39?v=Cell+Signaling+Technology+Inc
    Average 95 stars, based on 182 article reviews
    phosphorylated pka - by Bioz Stars, 2026-08
    95/100 stars

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    Immunohistochemical Staining Results Immunohistochemical staining and analysis of osteogenic-related proteins and signaling pathway markers in distraction areas at consolidation weeks 2, 4 and 6. Staining for Runx2, Osterix (Osx), osteopontin (OPN), osteocalcin (OCN), phosphorylated PKA (p-PKA), and phosphorylated CREB (p-CREB) across different treatment groups. Quantitative analysis shows positive staining area percentage (n = 3). Scale bar: 200 μm.

    Journal: Materials Today Bio

    Article Title: CGRP-releasing PLGA/nHA/GO composite microspheres enhance distraction osteogenesis via activation of the cAMP/PKA/CREB pathway

    doi: 10.1016/j.mtbio.2025.102181

    Figure Lengend Snippet: Immunohistochemical Staining Results Immunohistochemical staining and analysis of osteogenic-related proteins and signaling pathway markers in distraction areas at consolidation weeks 2, 4 and 6. Staining for Runx2, Osterix (Osx), osteopontin (OPN), osteocalcin (OCN), phosphorylated PKA (p-PKA), and phosphorylated CREB (p-CREB) across different treatment groups. Quantitative analysis shows positive staining area percentage (n = 3). Scale bar: 200 μm.

    Article Snippet: Membranes were blocked with TBST buffer containing 5 % skimmed milk at room temperature for 1–2 h. Subsequently, PVDF membranes were incubated overnight at 4 °C with specific primary antibodies including PKA antibody (Affinity, AF7746), phosphorylated PKA antibody (Affinity, AF7246), CREB antibody (Affinity, AF6188), phosphorylated CREB antibody (Affinity, AF3189), OPN antibody (Affinity, AF0227), OCN antibody (Affinity, DF12303) (Affinity Biosciences, USA); Runx2 antibody (Abcam, ab236639), Osx antibody (Abcam, ab209484), CyclinD1 antibody (Abcam, ab134175), GAPDH antibody (Abcam, ab181602) (Abcam, UK).

    Techniques: Immunohistochemical staining, Staining